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  1. Jalview
  2. JAL-4434

Import PTM features and metadata from Uniprot proteomics-ptm API

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    Details

    • Type: New Feature
    • Status: Open
    • Priority: Major
    • Resolution: Unresolved
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Detailed PTM metadata is available from the JSON endpoint:
      https://www.ebi.ac.uk/proteins/api/proteomics-ptm/A0A0P0XJ24

      In principle we could use the GFF3 content-type (curl -X GET --header 'Accept:text/x-gff' ') and import directly, but the JSON is much richer:

      {
        "accession": "A0A0P0XJ24",
        "entryName": "A0A0P0XJ24_ORYSJ",
        "sequence": "GKSGAKKKKPAAAASASAAATKSSPAATEPKAPTQSQPPAANGAAPHLVDPAVLLRRAHELKEEGNRLFQSRDYGGALRQYELALRLAPRGHPDRAVFHSNRAACLLQLRPVDHKAVAEECSLALQAEPLFPRALLRRARALEALGRHELALTDALALLALDPDHQDAVDLVHRLRSRILSPSSSASGSATSTPEPTSRPSPAALGASAVVAGLGPSLPARPFPKKPSPPPPPAQQQQSVPPMSKFNPSPPSPKLVPFSNSPPSSANASAAESSQKVTPTPLVPSSLSLKDKALMDKKVVTRSRPLKLVYDHDIRLAQMPEKCSFRTLREVVASRFPSSKAVLIKYKDADGDLVTITCSAELRLAESCVDIAGSEVIEDGARHGQKLPMLRLHIVEVSPDQEPPMPTEEEKLEQDNELLVKGEDNSPHASAAVVTDAEVTKQDVENVVAEAEQNTLTGKKDCGHAECKEAEIDDWLLQFADLFRNQVGVDADAHLDLHELGMELCSEALEETVTSEEAQALFEMAAAKFQEVAALALFNWGNVHMCAARKRIPLDESAPKKVMSAQLCTAYDWVRDRYALAGSKYEEALKIKPDFYEGLLALGQQHFETAKLHWSFALADKVDLSAWDSSQTFKLFDSAEHKMRAATEMWEKVEEQRMAELKEPSSEALKKRKKQHNADGQGELTPEEAAEQAAVMRQQIHLFWGNMLFERSQVEFKLDIADWKKNLDASIERFKLAGASESDISAVLKNHFSNTVSECEDKKIMTLDTGISQISNNIEDKCILES",
        "sequenceChecksum": "5941F741151D242B",
        "taxid": 39947,
        "features": [
          {
            "type": "PROTEOMICS_PTM",
            "begin": "246",
            "end": "254",
            "xrefs": [
              {
                "name": "Proteomes",
                "id": "UP000059680",
                "url": "https://www.uniprot.org/proteomes/UP000059680"
              }
            ],
            "evidences": [
              {
                "code": "ECO:0007829",
                "source": {
                  "id": "FNPSPPSPK",
                  "url": "https://www.ebi.ac.uk/pride/peptidome/peptidedetails?keyword=FNPSPPSPK&proteinAccession=A0A0P0XJ24"
                }
              }
            ],
            "peptide": "FNPSPPSPK",
            "unique": true,
            "ptms": [
              {
                "name": "Phosphorylation",
                "position": 7,
                "sources": [
                  "PTMeXchange"
                ],
                "dbReferences": [
                  {
                    "id": "PXD004939",
                    "properties": {
                      "Pubmed ID": "28054942",
                      "Confidence score": "Gold",
                      "PSM Score": "0.9994",
                      "Site q value": "0.0",
                      "Dataset ID": "PXD004939",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD004939:Rice_phos_ABA_12h_20per_F1_R2:scan:12866:FNPSPPS[Phospho]PK/2",
                      "Final site probability": "0.9784126",
                      "PSM Count (0.05 gFLR)": "30",
                      "Proforma": "FNPSPPS[Phospho]PK"
                    }
                  },
                  {
                    "id": "PXD005241",
                    "properties": {
                      "Pubmed ID": "28382632",
                      "Confidence score": "Gold",
                      "PSM Score": "0.9853",
                      "Site q value": "0.0170407241382277",
                      "Dataset ID": "PXD005241",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD005241:Rice_phos_mature_panicles__TiO2_25per_F2_R1_raw:scan:12925:FNPSPPS[Phospho]PK/2",
                      "Final site probability": "0.95002626",
                      "PSM Count (0.05 gFLR)": "5",
                      "Proforma": "FNPSPPS[Phospho]PK"
                    }
                  },
                  {
                    "id": "PXD004705",
                    "properties": {
                      "Pubmed ID": "28439285",
                      "Confidence score": "Gold",
                      "PSM Score": "0.9999",
                      "Site q value": "0.0024898044423765",
                      "Dataset ID": "PXD004705",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD004705:Rice_phos_BR_3h_20per_F1_R2:scan:12659:FNPSPPS[Phospho]PK/2",
                      "Final site probability": "0.95550444",
                      "PSM Count (0.05 gFLR)": "34",
                      "Proforma": "FNPSPPS[Phospho]PK"
                    }
                  },
                  {
                    "id": "PXD000923",
                    "properties": {
                      "Pubmed ID": "25074045",
                      "Confidence score": "Gold",
                      "PSM Score": "0.999",
                      "Site q value": "0.0055560314053066",
                      "Dataset ID": "PXD000923",
                      "Organism part": "Flower",
                      "Universal Spectrum Id": "mzspec:PXD000923:LHY_T5:scan:02407:FNPSPPS[Phospho]PK/2",
                      "Final site probability": "0.91025148767299",
                      "PSM Count (0.05 gFLR)": "3",
                      "Proforma": "FNPSPPS[Phospho]PK"
                    }
                  },
                  {
                    "id": "PXD002222",
                    "properties": {
                      "Pubmed ID": "26112675",
                      "Confidence score": "Gold",
                      "PSM Score": "0.9998",
                      "Site q value": "0.0",
                      "Dataset ID": "PXD002222",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD002222:Rice_leaf_0h_phospho_test2:scan:09105:FNPSPPS[Phospho]PK/2",
                      "Final site probability": "0.9803039",
                      "PSM Count (0.05 gFLR)": "24",
                      "Proforma": "FNPSPPS[Phospho]PK"
                    }
                  }
                ]
              }
            ]
          },
          {
            "type": "PROTEOMICS_PTM",
            "begin": "246",
            "end": "254",
            "xrefs": [
              {
                "name": "Proteomes",
                "id": "UP000059680",
                "url": "https://www.uniprot.org/proteomes/UP000059680"
              }
            ],
            "evidences": [
              {
                "code": "ECO:0007829",
                "source": {
                  "id": "FNPSPPSPK",
                  "url": "https://www.ebi.ac.uk/pride/peptidome/peptidedetails?keyword=FNPSPPSPK&proteinAccession=A0A0P0XJ24"
                }
              }
            ],
            "peptide": "FNPSPPSPK",
            "unique": true,
            "ptms": [
              {
                "name": "Phosphorylation",
                "position": 7,
                "sources": [
                  "PTMeXchange"
                ],
                "dbReferences": [
                  {
                    "id": "PXD019291",
                    "properties": {
                      "Pubmed ID": "33658224",
                      "Confidence score": "Gold",
                      "PSM Score": "1.0",
                      "Site q value": "0.0184438306538822",
                      "Dataset ID": "PXD019291",
                      "Organism part": "Anther",
                      "Universal Spectrum Id": "mzspec:PXD019291:S9588TPST_Fr1:scan:20749:[TMT6plex]FNPSPPS[Phospho]PK[TMT6plex]/2",
                      "Final site probability": "0.8460922634265026",
                      "PSM Count (0.05 gFLR)": "3",
                      "Proforma": "[TMT6plex]FNPSPPS[Phospho]PK[TMT6plex]"
                    }
                  }
                ]
              }
            ]
          },
          {
            "type": "PROTEOMICS_PTM",
            "begin": "422",
            "end": "441",
            "xrefs": [
              {
                "name": "Proteomes",
                "id": "UP000059680",
                "url": "https://www.uniprot.org/proteomes/UP000059680"
              }
            ],
            "evidences": [
              {
                "code": "ECO:0007829",
                "source": {
                  "id": "GEDNSPHASAAVVTDAEVTK",
                  "url": "https://www.ebi.ac.uk/pride/peptidome/peptidedetails?keyword=GEDNSPHASAAVVTDAEVTK&proteinAccession=A0A0P0XJ24"
                }
              }
            ],
            "peptide": "GEDNSPHASAAVVTDAEVTK",
            "unique": true,
            "ptms": [
              {
                "name": "Phosphorylation",
                "position": 5,
                "sources": [
                  "PTMeXchange"
                ],
                "dbReferences": [
                  {
                    "id": "PXD004939",
                    "properties": {
                      "Pubmed ID": "28054942",
                      "Confidence score": "Gold",
                      "PSM Score": "1.0",
                      "Site q value": "0.0",
                      "Dataset ID": "PXD004939",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD004939:Rice_phos_Nip_0h_20per_F1_R1:scan:17365:GEDNS[Phospho]PHASAAVVTDAEVTK/2",
                      "Final site probability": "0.977196147205192",
                      "PSM Count (0.05 gFLR)": "3",
                      "Proforma": "GEDNS[Phospho]PHASAAVVTDAEVTK"
                    }
                  },
                  {
                    "id": "PXD005241",
                    "properties": {
                      "Pubmed ID": "28382632",
                      "Confidence score": "Gold",
                      "PSM Score": "0.9967",
                      "Site q value": "0.0",
                      "Dataset ID": "PXD005241",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD005241:Rice_phos_young_panicles__TiO2_25per_F3_R1_raw:scan:28028:GEDNS[Phospho]PHASAAVVTDAEVTK/2",
                      "Final site probability": "0.9807527998278694",
                      "PSM Count (0.05 gFLR)": "10",
                      "Proforma": "GEDNS[Phospho]PHASAAVVTDAEVTK"
                    }
                  },
                  {
                    "id": "PXD004705",
                    "properties": {
                      "Pubmed ID": "28439285",
                      "Confidence score": "Gold",
                      "PSM Score": "1.0",
                      "Site q value": "0.0",
                      "Dataset ID": "PXD004705",
                      "Organism part": "Leaf",
                      "Universal Spectrum Id": "mzspec:PXD004705:Rice_phos_BR_3h_20per_F1_R3:scan:17196:GEDNS[Phospho]PHASAAVVTDAEVTK/2",
                      "Final site probability": "0.9877999822702022",
                      "PSM Count (0.05 gFLR)": "8",
                      "Proforma": "GEDNS[Phospho]PHASAAVVTDAEVTK"
                    }
                  }
                ]
              }
            ]
          },
          {
            "type": "PROTEOMICS_PTM",
            "begin": "255",
            "end": "276",
            "xrefs": [
              {
                "name": "Proteomes",
                "id": "UP000059680",
                "url": "https://www.uniprot.org/proteomes/UP000059680"
              }
            ],
            "evidences": [
              {
                "code": "ECO:0007829",
                "source": {
                  "id": "LVPFSNSPPSSANASAAESSQK",
                  "url": "https://www.ebi.ac.uk/pride/peptidome/peptidedetails?keyword=LVPFSNSPPSSANASAAESSQK&proteinAccession=A0A0P0XJ24"
                }
              }
            ],
            "peptide": "LVPFSNSPPSSANASAAESSQK",
            "unique": true,
            "ptms": [
              {
                "name": "Phosphorylation",
                "position": 7,
                "sources": [
                  "PTMeXchange"
                ],
                "dbReferences": [
                  {
                    "id": "PXD000923",
                    "properties": {
                      "Pubmed ID": "25074045",
                      "Confidence score": "Bronze",
                      "PSM Score": "0.998",
                      "Site q value": "0.0202587687670536",
                      "Dataset ID": "PXD000923",
                      "Organism part": "Flower",
                      "Universal Spectrum Id": "mzspec:PXD000923:LHY_T6:scan:12749:LVPFSNS[Phospho]PPSSANASAAESSQK/3",
                      "Final site probability": "0.7172018572236077",
                      "PSM Count (0.05 gFLR)": "1",
                      "Proforma": "LVPFSNS[Phospho]PPSSANASAAESSQK"
                    }
                  }
                ]
              }
            ]
          },
          {
            "type": "PROTEOMICS_PTM",
            "begin": "277",
            "end": "290",
            "xrefs": [
              {
                "name": "Proteomes",
                "id": "UP000059680",
                "url": "https://www.uniprot.org/proteomes/UP000059680"
              }
            ],
            "evidences": [
              {
                "code": "ECO:0007829",
                "source": {
                  "id": "VTPTPLVPSSLSLK",
                  "url": "https://www.ebi.ac.uk/pride/peptidome/peptidedetails?keyword=VTPTPLVPSSLSLK&proteinAccession=A0A0P0XJ24"
                }
              }
            ],
            "peptide": "VTPTPLVPSSLSLK",
            "unique": true,
            "ptms": [
              {
                "name": "Phosphorylation",
                "position": 12,
                "sources": [
                  "PTMeXchange"
                ],
                "dbReferences": [
                  {
                    "id": "PXD000923",
                    "properties": {
                      "Pubmed ID": "25074045",
                      "Confidence score": "Bronze",
                      "PSM Score": "1.0",
                      "Site q value": "0.039115499603902",
                      "Dataset ID": "PXD000923",
                      "Organism part": "Flower",
                      "Universal Spectrum Id": "mzspec:PXD000923:LHY_T4:scan:08926:VTPTPLVPSSLS[Phospho]LK/2",
                      "Final site probability": "0.602362602131413",
                      "PSM Count (0.05 gFLR)": "1",
                      "Proforma": "VTPTPLVPSSLS[Phospho]LK"
                    }
                  }
                ]
              }
            ]
          }
        ]
      }

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            Reporter:
            jprocter James Procter
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              Created:
              Updated: