LOCUS       pJET_2.1                2974 bp    DNA     circular     22-Jan-2013
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     misc_feature    305..323
                     /label="Promoter T7_promoter"
                     /note="Pr_T7"
     misc_feature    1148..1767
                     /label="Origin pBR322_replication_origin"
                     /note="Ori_pBR322"
     misc_feature    1922..2782
                     /label="Selection ampicillin_resistance_gene_ORF"
                     /note="amp"
     misc_feature    2824..2852
                     /label="Promoter ampicillin_resistance_gene(b-lactamase)promoter"
                     /note="Pr_amp"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc 
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc 
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact 
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt 
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc 
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt 
      361 ttcagcaaga tatctttcta gaagatctcc tacaatattc tcagctgcca tggaaaatcg 
      421 atgttcttct tttattctct caagattttc aggctgtata ttaaaactta tattaagaac 
      481 tatgctaacc acctcatcag gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact 
      541 ctcatgaaaa ctacgagcta aatattcaat atgttcctct tgaccaactt tattctgcat 
      601 tttttttgaa cgaggtttag agcaagcttc aggaaactga gacaggaatt ttattaaaaa 
      661 tttaaatttt gaagaaagtt cagggttaat agcatccatt ttttgctttg caagttcctc 
      721 agcattctta acaaaagacg tctcttttga catgtttaaa gtttaaacct cctgtgtgaa 
      781 attattatcc gctcataatt ccacacatta tacgagccgg aagcataaag tgtaaagcct 
      841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg ccaattgctt 
      901 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 
      961 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     1021 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     1081 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     1141 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     1201 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     1261 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     1321 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     1381 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     1441 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     1501 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     1561 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     1621 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     1681 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     1741 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     1801 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     1861 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     1921 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     1981 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     2041 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     2101 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     2161 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     2221 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     2281 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     2341 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     2401 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     2461 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     2521 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     2581 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     2641 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     2701 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     2761 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     2821 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     2881 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 
     2941 cattaaccta taaaaatagg cgtatcacga ggcc
//